Marcos Senesi, 29, has joined as a free agent, while Jan Paul van Hecke, 26, has arrived from Brighton, putting fresh focus on where the 25-year-old Dutchman fits into Tottenham's defensive plans.
At the same time, 19-year-old talent Luka Vuskovic is pushing for a starting role after an impressive loan spell at Hamburg.
According to Dutch outlet De Telegraaf, talks over a new contract between Van de Ven and Spurs have stalled.
The same reports claim that both Liverpool and Barcelona are monitoring the situation and could move to strengthen their own central defence by taking advantage of the uncertainty.
Van de Ven is under contract at Tottenham until the summer of 2029, giving the club time to resolve his situation, but the stalled talks combined with two fresh defensive arrivals and a highly-rated teenager pushing for involvement leave his position looking less secure than it did at the start of the summer.
The Dutchman has been one of Tottenham's most important players since his £43m move from Wolfsburg in 2023, forming a key partnership at the back during a period in which the club has otherwise struggled defensively, conceding 122 goals across the past two Premier League seasons combined.
Spurs' decision to add Senesi and Van Hecke despite Van de Ven's continued presence in the squad suggests the club is building genuine depth at centre-back rather than viewing any single signing as a direct replacement, but a stalled contract renewal for one of the club's most valuable defensive assets will inevitably fuel speculation over his long-term future regardless of how the depth chart is structured.