England will run a late fitness test on Guehi on Friday to determine whether the centre-back can feature in Saturday's World Cup quarter-final against Norway, with head coach Thomas Tuchel also confirming that Quansah will sit out the game after being handed a two-match ban.
Guehi has a hamstring strain picked up during the 3-2 win over Mexico in the last 16, having initially been thought to be muscular fatigue. The Manchester City defender is desperate to prove his fitness and remains optimistic, but if he cannot train fully with the squad on Friday there will be major doubt over his involvement. The issue is not considered serious, though with Norway's Erling Haaland - the tournament's top scorer with seven goals - to contend with, Tuchel can ill afford to take risks in defence.
There was better news at right-back, where Reece James returned to full training for the first time since injuring his hamstring in the goalless draw with Ghana. James did not complete the entire session but is expected to be available against Norway providing he shows no ill effects.
Declan Rice, meanwhile, missed a second successive day of training with a sickness bug that has aggravated an existing neural issue affecting his hamstring and lower back, with England taking steps to prevent the illness spreading further through the camp.
Quansah's absence adds to the selection headache at right-back. The Bayer Leverkusen defender was sent off in the 54th minute against Mexico for a high challenge on Jesus Gallardo, classed as serious foul play, meaning Fifa's disciplinary committee added an extra match to the automatic one-game suspension.
Quansah, 23, will miss Saturday's game and a potential semi-final, but would be available for the final in New Jersey on 19 July should England reach it. The FA has no route to appeal under tournament regulations, though BBC Sport understands strong representations were made to Fifa over the process, with the referee shown a still image and slow-motion replays of the incident before viewing it in real time on the pitchside screen - a sequence England's camp believe could have led to "outcome bias".
Quansah had deputised for James against Mexico, with Djed Spence used only as a substitute after a minor fitness issue of his own. Assistant coach Anthony Barry said the news was hard to take.
"Disappointing, not with the decision, just the fact that we lose a good player," he said.
"He was excellent in training, and of course we have some injuries in that position, so it looked like a space had opened up for Jarell.
"But the decision's been made - we won't waste any more energy on it. Overall for us, we lose a good player for two games, but it's just another hurdle that we have to overcome."
Winger Bukayo Saka echoed that sentiment.
"It is incredibly frustrating for us, and for him," Saka said. "It is what it is, we are not here to complain, we are here to adapt and to pick a team that is ready to beat Norway."